Grab, Manipulate and Watch Single DNA Molecule Replication

At the 66th Annual Biophysical Society Annual Meeting in San Francisco, held at the Moscone Center, I presented my PhD work titled "Grab, Manipulate and Watch Single DNA Molecule Replication."

The presentation showcased our innovative approach that combines two independent yet correlative measurements: force data from optical tweezers and imaging data from fluorescence microscopy. This dual-measurement strategy provides unprecedented insights into the molecular mechanisms of DNA replication.

By grabbing and manipulating single DNA molecules while simultaneously watching their replication in real-time, we developed a powerful methodology for understanding how replication machinery operates at the molecular level. This work laid the foundation for what would later become the C-DAM (Correlated Dynamics and Activity Mapping) technology.

The conference provided an excellent platform to present this work to the broader biophysics community and receive valuable feedback from experts in single-molecule biophysics, DNA replication, and force spectroscopy.