A kind of albumin binding peptide-human ciliary nerve nutrition factor fusion protein and its preparation method and application/一种白蛋白结合肽-人睫状神经营养因子融合蛋白及其制备方法和应用
Abstract
The present invention relates to a kind of albumin binding peptide human ciliary nerve nutrition factor fusion proteins and its preparation method and application, the fusion protein includes albumin binding peptide, human ciliary nerve nutrition factor and flexible peptide fragment, the fusion protein not only extends the Half-life in vivo of recombination human ciliary neurotrophy factor, also maintains its activity.The fusion protein can be in E. coli, and purifying process is simple, can be used for the fusion protein sterling that large-scale industrial production prepares pharmaceutical grade.With recombinate homologous human ciliary nerve nutrition factor (rhCNTF) in itself compared with, the ABD CNTF fusion proteins that the present invention is obtained show serum half-life and significantly extend, and the Bioactivity of human ciliary nerve nutrition factor is kept, so as to improve pharmacokinetics and drug effect.
Longfu Xu
PhD in Biophysics
My research topics focus on exploring DNA-proteins interactions using single-molecule visualization and manipulation techniques, with a big passion for understanding life with physical principles.